Table 1. Oligonucleotides sequence and their molar extinction coefficients used in this study.
ID | Name | Sequencea | ϵ (M−1cm−1) 260 nmb | Bufferc |
---|---|---|---|---|
1 | AT Duplex | 5′-ATA TAT ATC CCC ATA TAT AT-3′ | 205700 | A |
2 | GC Duplex | 5′-GCG CGC GCT TTT GCG CGC GC-3′ | 166300 | A |
3 | A/T Duplex | 5′-AAA AAA AAC CCC TTT TTT TT-3′ | 191300 | A |
4 | H-Tel Duplex | 5′-GGG TTA GGG TTT TCC CTA ACC C-3′ | 202200 | A |
5 | AT Triplex | 5′-AAA AAA AAC CCC TTT TTT TTC CCC TTT TTT TT-3′ | 284900 | C |
6 | GC Triplex | 5′-CCC CCC CCT TTT GGG GGG GGT TTT CCC CCC CC-3′ | 261600 | B |
7 | H-Tel i-Motif | 5′-CCC TAA CCC TAA CCC TAA CCC T-3′ | 193700 | B |
8 | H-Tel Quadruplex | 5′-AGG GTT AGG GTT AGG GTT AGG G-3′ | 228500 | A |
9 | c-Myc Quadruplex | 5′-TGA GGG TGG GTA GGG TGG GTA A-3′ | 228700 | A |
10 | c-Myc i-Motif | 5′-TTC CCC ACC CTC CCC ACC CTC CCC TAA-3′ | 222200 | B |
11 | A/U RNA duplex | 5′-rArArA rArArA rArArC rCrCrC rUrUrU rUrUrU rUrU-3′ | 202900 | C |
12 | DNA-RNA duplex | 5′-GGG TTA GGG TTT TrCrC rCrUrA rArCrC rC-3′ | 202900 | A |
aThe strand orientation is 5′ to 3′ from left to right, and ‘r’ denotes a ribonucleotide.
bMolar nearest neighbor extinction coefficients of the single-strand at 260 nm.
cBuffer conditions: A = 6 mM K2HPO4, 4 mM KH2PO4, 15 mM KCl, pH 7. 2; B = 1 mM K2HPO4, 9 mM KH2PO4, 15 mM KCl, pH 6.2; C = 6 mM K2HPO4, 4 mM KH2PO4, 15 mM KCl, 1 mM MgCl2, pH 7. 2.