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. 2018 Feb 13;46(7):3468–3486. doi: 10.1093/nar/gky098

Figure 8.

Figure 8.

Increased DSB and declined recombination in Pold3+/− spermatocytes with age. (A) Representative immunofluorescence images showing co-immunostaining of γH2AX and SYCP3 in Pold3+/+ and Pold3+/− spermatocytes, and accumulation of γH2AX in pachytene spermatocytes shown at higher magnification. Scale bar, 20 μm. (B and C) Statistical results of (A). n = 3 mice for each group, and 50 spermatocytes counted for each mouse. (D) Representative immunofluorescence images showing co-immunostaining of RAD51 and SYCP3. Scale bar, 20 μm. (E and F) Statistical results of (D). n = 3 mice for each group, and 50 spermatocytes counted for each mouse. (G) Representative immunofluorescence images showing co-immunostaining of MLH1 and SYCP3. Scale bar, 20 μm. (H and I) Statistical results of (G). Mean ± SEM. *P < 0.05; **P < 0.01.