Figure 6.

Regulation of Ces2b, a class I male-biased gene, by GH pulse–activated STAT5. (a) DHS analysis at a male-biased STAT5 binding site in the Ces2b promoter [Fig. 3(c); Supplemental Table 1] carried out for intact and hypophysectomized male and female mouse livers, with a time course of GH treatment (left) as in Fig. 2. Chromatin accessibility at this DHS was significantly higher in STAT5-high than in STAT5-low livers (right). (b and c) RT-qPCR analysis of Ces2b hnRNA and Ces2b mRNA. Data shown in the left set of graphs for (a)–(c), that is, the hypophysectomy series, are mean ± SEM values for n = 5 to 6 livers per group, except for the intact male group, where n = 18. The right set of graphs (STAT5-high, STAT5-low) are based on n = 9 to 12 livers per group. Relative DHS activity and expression level of the intact male (left) and the STAT5-high groups (right) are set to 1. Significance values by ANOVA or two-tailed t test are marked as described in the Fig. 2 legend.