Figure 4.

DKO brown preadipocytes showed an impaired p62-TOM20 colocalization upon 4 hour of CCCP treatment. (a) Representative immunofluorescence of TOM20, p62, and 4′,6-diamidino-2-phenylindole (DAPI) from brown preadipocytes obtained from WT or DKO mice under control conditions or CCCP treatment. In the CCCP treatment of both cell lines, two different regions are amplified in the insets. (b) After use of the Coloc2 plug-in (as explained in the Materials and Methods section), colocalization Manders coefficient after CCCP treatment is represented in the graph. Results are presented as mean ± standard error of the mean. Statistical significance was assessed by two-tailed Student t test; ***P < 0.001 between WT (n = 3) and DKO (n = 3) cells treated with CCCP for 4 hours.