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. 2017 Oct 10;159(1):323–340. doi: 10.1210/en.2017-00738

Figure 5.

Figure 5.

Impaired brown fat thermogenesis and mitochondrial fission mechanisms upon cold exposure in DKO mice. (a) Representative PET images of iBAT 18F-FDG uptake from 3-month-old WT (n = 2) and DKO (n = 2) mice at room temperature (RT; left panels) or at 4°C (right panels). (b) PET quantification shown as standard uptake value (SUV). Results are presented as mean ± standard error of the mean (SEM). Statistical significance was assessed by two-way analysis of variance (ANOVA), followed by the Tukey test; &&&P < 0.001 between WT RT and DKO RT groups; $$$P < 0.001 between WT 4°C and DKO 4°C groups; and ***P < 0.001 between WT RT and WT 4°C groups. (c) Representative plots of cold-exposure analysis showing the temperature from 0 to 12 hours comparing 3-month-old (WT, n = 8; n = 7 DKO) and 12-month-old (WT, n = 4; DKO, n = 4) mice. Results are presented as mean ± SEM. Statistical significance was assessed by two-way ANOVA, followed by the Tukey test; *P < 0.05 and **P < 0.01 between 3-month-old WT and DKO groups; ##P < 0.01 and ###P < 0.001 between 12-month-old WT and DKO groups. (d) BMP7 plasma levels from 12-month-old WT [control (Ct), n = 8 ; 4 hours (4h), n = 8 ] and DKO (Ct, n = 4; 4h, n = 4) mice. (e) T3 plasma levels from 12-month-old WT (Ct, n = 9; 4h, n = 4) and DKO (Ct, n = 6; 4h, n = 4) mice. (f) Representative Western blots showing different regulators of mitochondrial dynamics and brown fat functionality of iBAT from 12-month-old WT and DKO mice at thermoneutrality (WT, n = 4; DKO, n = 3) or after 4 hours of 4°C exposure (WT, n = 4; DKO, n = 3) and its corresponding quantification. Results are presented as mean ± SEM. Statistical significance was assessed by one-way ANOVA (BMP7, T3 plasma levels, p-Drp1/Drp1, PGC1-α, UCP1, and BMP7) or two-way ANOVA (OPA-1), followed by the Tukey test; *P < 0.05, **P < 0.01, and ***P < 0.001 between WT Ct and WT 4h groups; ##P < 0.01 between DKO Ct and DKO 4h; $P < 0.05 and $$P < 0.01 between WT 4h and DKO 4h; &P < 0.05 between WT Ct and DKO Ct; and ^P < 0.05 between L-OPA-1 WT 4h and S-OPA-1 WT 4h.