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. 2017 May 17;158(8):2618–2633. doi: 10.1210/en.2017-00032

Figure 4.

Figure 4.

Effect of HOXA10 knockdown in decidual cells on invasion of trophoblast cells. Trophoblast cell lines (a) ACH-3P and (b) JEG3 were treated with diluted (25%) and neat (100%) conditioned medium and loaded onto the Matrigel. After 24 hours of incubation, cells present in the lower chamber were counted. The different types of conditioned media used are shown on the x-axis. Predecidual refers to conditioned medium from endometrial stromal cells treated with steroids for 8 days; decidual refers to conditioned medium from endometrial stromal cells treated with steroids for 21 days. Scrambled siRNA and HOXA10 siRNA samples are the conditioned medium obtained from decidual cells (day 21 of steroid treatment) after 72 hours of transfection with respective siRNAs. Control represents nontransfected control cells. Values on the y-axis represent the fold change in the number of invading cells with respect to control medium (taken as 1). Each dot represents value obtained from one experiment. Mean values that are statistically significant (n = 4) are denoted by bars, along with the respective P values.