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. 2018 Dec 6;13(12):e0208453. doi: 10.1371/journal.pone.0208453

Fig 1. Mouse brain mitochondria have lower respiratory flux at a hypothermic temperature relative to physiological temperature.

Fig 1

(A) State II (through complex I; pyruvate, malate and glutamate-fueled), state III (through complex I, ADP-fueled), state III (through complex II, succinate-fueled in the presence of rotenone), and complex IV respiratory rates from permeabilized brain mitochondria treated at physiological temperature (37°C; white bars) or a hypothermic temperature (28°C; black bars) normalized per mg protein in brain. (B) Comparison of complex I and II fueled FCCP-uncoupled respiration rates between experimental temperatures. (C) Comparison of non-mitochondrial oxygen consumption between experimental temperatures. Data are mean ± SEM. Numbers in parenthesis indicate n. Asterisks (*) indicate significant differences between physiological and hypothermic temperatures (p < 0.05; see Results section for statistical tests).