Table 2.
Comparison of Giardia lamblia sensitivity between whole stool samples and stool samples stored on Whatman protein saver cards
Application method | DNA extraction method | High parasite load* (N = 10) | Low parasite load† (N = 10) | All samples (N = 20) |
---|---|---|---|---|
Sensitivity (95% CI) | Sensitivity (95% CI) | Sensitivity (95% CI) | ||
Whole stool directly on Whatman cards | Qiagen | 100% (95% CI: 69%, 100%) | 90% (95% CI: 56%, 100%) | 95% (95% CI: 75%, 100%) |
Chelex | 100% (95% CI: 69%, 100%) | 60% (95% CI: 26%, 88%) | 80% (95% CI: 56%, 94%) | |
Stool suspension on Whatman cards | Qiagen | 100% (95% CI: 69%, 100%) | 70% (95% CI: 35%, 93%) | 85% (95% CI: 62%, 97%) |
Chelex | 100% (95% CI: 69%, 100%) | 60% (95% CI: 26%, 87%) | 80% (95% CI: 56%, 94%) |
CI = confidence interval.
* Whole stool ct value < 30.
† Whole stool ct value ≥ 30.