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. 2018 Nov 5;99(6):1613–1618. doi: 10.4269/ajtmh.18-0538

Table 4.

Comparison of Cryptosporidium spp. sensitivity between whole stool samples and stool samples stored on Whatman protein saver cards

Application method DNA extraction method High parasite load* (N=10) Low Parasite Load† (N=10) All samples (N = 20)
Sensitivity (95% CI) Sensitivity (95% CI) Sensitivity (95% CI)
Whole stool directly on Whatman cards Qiagen 80% (95% CI: 44%, 97%) 0% (95% CI: 0%, 31%) 40% (95% CI: 19%, 64%)
Chelex 50% (95% CI: 19%, 81%) 0% (95% CI: 0%, 31%) 25% (95% CI: 9%, 49%)
Stool suspension on Whatman cards Qiagen 70% (95% CI: 35%, 93%) 0% (95% CI: 0%, 31%) 35% (95% CI: 15%, 59%)
Chelex 70% (95% CI: 25%, 93%) 0% (95% CI: 0%, 31%) 35% (95% CI: 15%, 59%)

CI = confidence interval.

* Whole stool ct value < 30.

† Whole stool ct value ≥ 30.