Table 4.
Application method | DNA extraction method | High parasite load* (N=10) | Low Parasite Load† (N=10) | All samples (N = 20) |
---|---|---|---|---|
Sensitivity (95% CI) | Sensitivity (95% CI) | Sensitivity (95% CI) | ||
Whole stool directly on Whatman cards | Qiagen | 80% (95% CI: 44%, 97%) | 0% (95% CI: 0%, 31%) | 40% (95% CI: 19%, 64%) |
Chelex | 50% (95% CI: 19%, 81%) | 0% (95% CI: 0%, 31%) | 25% (95% CI: 9%, 49%) | |
Stool suspension on Whatman cards | Qiagen | 70% (95% CI: 35%, 93%) | 0% (95% CI: 0%, 31%) | 35% (95% CI: 15%, 59%) |
Chelex | 70% (95% CI: 25%, 93%) | 0% (95% CI: 0%, 31%) | 35% (95% CI: 15%, 59%) |
CI = confidence interval.
* Whole stool ct value < 30.
† Whole stool ct value ≥ 30.