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. 2018 Oct 22;99(6):1430–1439. doi: 10.4269/ajtmh.17-0948

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© The American Society of Tropical Medicine and Hygiene

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Figure 1. — Side-by-side comparison of virus reduction neutralization test (VRNT) and focus reduction neutralization test (FRNT) methods. In this figure, day 1 is considered plate seeding rather than assay day 1. Differences include plate format (96-well for VRNT and 24-well for FRNT), infection time and secondary antibody and detection. Virus reduction neutralization test detection relies on fluorescent stained cells and cytometer counting; FRNT relies on peroxidase substrate and foci formation for manual foci counting. This figure appears in color at www.ajtmh.org.