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. 2018 Oct 22;99(6):1430–1439. doi: 10.4269/ajtmh.17-0948

Table 1.

Design of experiment factors and levels investigated for DEN2

Factor Whole plot or subplot Level 1 Level 2 Level 3 Level 4
A. Plate incubation temperature Whole plot Ambient 37°C N/A N/A
B. Primary Ab incubation time and temperature Whole plot 60 minutes/37°C Overnight/4°C N/A N/A
C. Secondary Ab incubation time Whole plot 30 minutes 90 minutes N/A N/A
D. Cell seeding density Subplot 1 × 104 cells 3 × 104 cells N/A N/A
E. Multiplicity of infection Subplot 0.01 0.1 N/A N/A
F. Neutralization mix incubation-time/temp Subplot 30 minutes/37°C Overnight/4°C 60 minutes/37°C 60 minutes/ambient
G. Primary Ab concentration Subplot 1 µg/mL 10 µg/mL N/A N/A
H. Secondary Ab dilution Subplot 1:250 1:1,000 N/A N/A

N/A = not applicable.