Table 2.

Design of experiment factors, levels and constant factors investigated for DEN1

	Level 1	Level 2
DOE factors
A. Multiplicity of infection	0.01	0.1
B. Primary Ab concentration	1 µg/mL	2.5 µg/mL
C. Secondary Ab dilution	1:250	1:1,000
Constant factors
D. Neutralization mix incubation temperature	Ambient
E. Primary Ab incubation time and temperature	60 minutes/37°C
F. Secondary Ab incubation time	60 minutes/37°C
G. Cell seeding density	30,000 cells/well
H. Neutralization time and temperature	30 minutes/37°C

DOEs = design of experiments.