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Summary of optimal conditions for DEN2 design of experiments
| Factor | Conclusion |
|---|---|
| A: Neutralization mixture plate incubation temperature | Ambient |
| B: Incubation time and temperature of primary antibody | 60 minutes at 37°C |
| C: Secondary antibody incubation time | 70 minutes |
| D: Cell seeding density | 3 × 104 |
| E: Multiplicity of infection | 0.01 |
| F: Neutralization mixture time and temperature | 30 minutes at 37°C |
| G: Concentration of primary antibody | 1 µg/mL |
| H: Secondary antibody dilution | 1:1,000 |
