Fig 8. Transcriptional regulation of PhoP of filipin biosynthesis is indirect.

EMSAs of protein extract binding to the promoter regions of filA1, filA2, filA3, filRF, filG and phoURP. The arrows indicate the DNA–protein complexes. All experiments were carried out with 0.08 ng/μl labeled DNA probe. The probes were incubated for 30 min at 30°C with 30 μg of protein extract prepared from cultures of S. filipinensis and S. filipinensis ΔphoRP grown for 48 h on YEME medium. Binding of 0.4 μM pure GST-PhoP^DBD was used as control. Promoter regions are indicated at the top of each panel. C: control without protein.