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. 2018 Dec 6;13(12):e0208316. doi: 10.1371/journal.pone.0208316

Fig 6. Hyperoxidation of 2Cys-Prdx is altered in patients BTM.

Fig 6

Representative western blot for hyperoxidation state of 2CysPrdx. Erythroid cells of BTI (A) and BTM (B) patients were lysed in the presence of 200 uM of Nethylmaleimide (NEM) to prevent further sample oxidation. Hyperoxidation levels were measured using 50 μg of total protein from each sample running in a 12% (w/v) reducing SDS–PAGE. The intensity of the bands was measured using GAPDH as the endogenous control. Quantitative analyzes were performed by densitometry using ImageJ Software [35]. Results are presented as mean and standard error (± SEM). Statistical significance: *p < 0.05.