Table 1. Details on models and corresponding species used in in silico essentiality simulations.

Phylum	Species	Model ID	# Reactions	# Metabolites	% ORFs	Reference
Firmicutes	Bacillus subtilis	iYO844	1020	988	21%	[31]
	Clostridium beijerinckii NCIMB 8052	iCB925	938	881	18%	[32]
	Staphylococcus aureus N315	iSB619	641	571	24%	[33]
Proteobacteria	Escherichia coli K12	iAF1260	2077	1039	29%	[34]
	Escherichia coli W (ATCC9637)	iCA1273	2477	1111	27%	[35]
	Helicobacter pylori 16695	iIT341	476	485	21%	[36]
	Klebsiella pneumoniae MGH 78578	iYL1228	1970	1658	24%	[37]
	Pseudomonas putida KT2440	iJN746	950	911	14%	[38]
	Salmonella typhimurium LT2	STM_v1.0	2201	1119	28%	[39]
	Shewanella oneidensis MR-1	iSO783	774	634	15%	[40]
Actinobacteria	Mycobacterium tuberculosis H37Rv	iNJ661	939	828	15%	[41]
Chloroflexi	Dehalococcoides ethenogenes	iAI549	518	549	27%	[42]
Cyanobacteria	Synechocystis sp. PCC6803	iJN678	863	795	21%	[43]
Thermotogales	Thermotoga maritima MSB8	(None)	562	503	25%	[44]
Euryarchaeota	Methanosarcina barkeri str. Fusaro	iAF692	476	485	14%	[45]