Table 2. Large-scale essentiality assays used in this study, number of essential genes in each and respective original reference of publication.

Species name	Number of essential genes		Reference a
Acinetobacter baylyi ADP1		499	[12]
Bacillus subtilis 168		271	[50]
Bacteroides fragilis 638R		547	[51]
Bacteroides thetaiotaomicron VPI-5482		325	[52]
Burkholderia pseudomallei K96243		505	[53]
Burkholderia thailandensis E264		406	[54]
Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819		228	[55]
Caulobacter crescentus		480	[56]
Escherichia coli MG1655 I		609	[10]
Escherichia coli MG1655 II		296	[57]
Francisella novicida U112		392	[11]
Haemophilus influenzae Rd KW20		642	[58]
Helicobacter pylori 26695		323	[59]
Methanococcus maripaludis S2		519	[60]
Mycobacterium tuberculosis H37Rv		614	[61]
Mycobacterium tuberculosis H37Rv II		771	[62]
Mycobacterium tuberculosis H37Rv III		687	[63]
Mycoplasma genitalium G37		381	[64]
Mycoplasma pulmonis UAB CTIP		310	[65]
Porphyromonas gingivalis ATCC 33277		463	[66]
Pseudomonas aeruginosa PAO1		117	[67]
Pseudomonas aeruginosa UCBPP-PA14		335	[68]
Salmonella enterica serovar Typhi		353	[14]
Salmonella enterica serovar Typhi Ty2		358	[69]
Salmonella enterica serovar Typhimurium SL1344		353	[69]
Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S		105	[70]
Salmonella typhimurium LT2		230	[71]
Shewanella oneidensis MR-1		403	[72]
Sphingomonas wittichii RW1		535	[73]
Staphylococcus aureus N315		302	[74]
Staphylococcus aureus NCTC 8325		351	[15]
Streptococcus pneumoniae		244	[75]
Streptococcus pyogenes MGAS5448		227	[76]
Streptococcus pyogenes NZ131		241	[76]
Streptococcus sanguinis		218	[77]
Vibrio cholerae N16961		779	[13]

^a The corresponding annotated data was obtained from the DEG database [17].