Fig 5. vhs exhibits differential activity against late virus transcripts in the absence of VP22.

(A) The time course described in Fig 4C was analysed by qRT-PCR for representative IE (ICP27 and ICP22), E (TK) and late (VP22, vhs and gC) transcripts. Note that the results are represented here as mean ΔCT values at each time point. The mean and ± standard error for n = 3 is shown. (B) Total lysates harvested at the same time as the RNA samples in Fig 4C were analysed by SDS-PAGE and Western blotting with antibodies for the indicated proteins. (C) HFFF cells were infected with Wt HSV1 at a multiplicity of 2. At 6 hours, the cells were either harvested for total RNA, or actinomycin D (5 μg/ml) was added and the infection left for a further 4 hours before harvesting total RNA. qRT-PCR was carried out on all samples for the indicated virus transcripts. Note that in this graph, results are expressed as the log₂ FC to the samples harvested at 6 hours (ΔΔCT). The mean and ± standard error for n = 3 is shown. Statistical analysis was carried out using an unpaired, two-way student’s t test. ns, p > 0.05. * p < 0.05. ** p < 0.01. *** p < 0.001.