Fig 7. Nuclear retention of late virus transcripts in the absence of VP22.

(A) HFFF cells infected with Wt, Δvhs or Δ22 viruses at a multiplicity of 2 were fixed 12 hours after infection and processed for immunofluorescence with an antibody to PABP (green). Nuclei were counterstained with DAPI (blue). (B) HFFF cells infected with Wt, Δvhs or Δ22 viruses at a multiplicity of 2 were fixed at 8 or 16 hours after infection and processed for mRNA FISH with probes specific to an IE (ICP27), E (TK) or late transcript (gC) (all in red). Nuclei were counterstained with DAPI (blue). (C) As for B, but cells fixed at 16 hours were processed for mRNA FISH with a probe specific to the late transcript VP16, or the spliced IE transcript ICP0 (both in red). Nuclei were counterstained with DAPI (blue). Scale bar = 20 μm.