Fig 2. Effects of IL-6 on IKr activation and inactivation kinetics in HEK-hERG cells.
A, Plots of IKr tail I/I+60mV for control and in the presence of IL-6 alone and IL-6+IL-6R. Compared to control IKr currents (circle, n = 21), the normalized tail currents show that IKr activates at more negative potentials in the presence IL-6 (square, n = 18) and IL-6+IL-6R (triangle, n = 9). B, Plots of activation time course (τactivation) and voltage relationship for basal IKr (circle, n = 21), and in the presence of IL-6 (square, n = 18) or IL-6+IL-6R (triangle, n = 9). The time course of inactivation (τinactivation) was examined using the protocol shown in C. D, representative IKr current traces recorded under basal conditions, and in the presence of IL-6 (E) or IL-6+IL-6R (F). G, graph of the voltage-dependence of the τinactivation during basal IKr (circle, n = 8), +IL-6 (square, n = 7), +IL-6+IL-6R (triangle, n = 5) conditions.