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. 2018 Dec 6;13(12):e0208321. doi: 10.1371/journal.pone.0208321

Fig 6. Transcript and protein expression of IL-6R in guinea pig heart.

Fig 6

A, qRT-PCR assay for detection of IL-6R mRNA expression in guinea-pig heart. Compared to control (Lane 1), IL-6R (120 bp) mRNA expression was detected in both atria (Lane 2) and ventricles (Lane 3) consistent with an endogenous IL-6R expression in guinea pig heart. B, Western blot of IL-6R from guinea-pig ventricular cell lysates. The blot was probed with anti-IL6Rα H-7. The band around 80 kDa (Fig 6B, Lane 1) represents IL-6Rα which was absent when IL-6Rα Ab was pre-incubated with its own blocking peptide (Fig 6B, Lane 2).