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. 2018 Dec 6;13(12):e0208556. doi: 10.1371/journal.pone.0208556

Fig 3. Inhibitory effects of AnT Fr on HUVECS proliferation i.e. tubes formation.

Fig 3

Cells were allowed to proliferate in EBM2 media in the presence and absence of various concentrations i.e. 5, 30, 60 and 100 μg/mL (nontoxic range) of AnT Fr and tubes formation by HUVECs in Matrigel was observed with the help of a phase contrast inverted microscope (Nikon, Tokyo, Japan) and photographed with Scion Image software (NIH, ML, USA) at 5 random sites. Total tubes length was then calculated for each group. Experiments were performed in triplicate. Data are the mean values ± SEM. (* p < 0.05 and ** p < 0.01 as compared to control).