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. 2018 Dec 6;13(12):e0208556. doi: 10.1371/journal.pone.0208556

Fig 5. Viability of 3T3-L1 adipocytes under various concentrations of AnT Fr determined by MTT assay.

Fig 5

3T3-L cells were cultured in DMEM media in 96 well microtiter plate and after attachment different concentrations of AnT Fr were added to different wells contained same number of cells. Cells without treatment was taken as control. Cell viability was calculated for each group by checking the absorbance of the dissolved formazan crystals in DMSO after addition of MTT reagent. Safe and toxic concentrations were determined. Data are expressed as mean ± SEM of three different experiments. (* p < 0.05 and ** p < 0.01 as compared to control).