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. 2018 Dec 6;13(12):e0208556. doi: 10.1371/journal.pone.0208556

Table 1. Gene-specific primers along with their product size and gene bank accession number used for PCR for antiangiogenic activities determination.

Gene Gene Bank Accession No. Primer sequence Size (bp)
VEGFR-2 NM_002253.2 F -AGG TTG CGT GTT CTT CGA GT
R -CCC AAA GTG CTG GGT TTT TA
934
PI3K NM_006218.2 F -CGT GTG CCA TTT GTT TTG AC
R -TCA AAC CCT GTT TGC GTT TAC
536
NF-kB NM_001145138.1 F -TGG TCA GCT CCC TTC TCT GT
R -GCC AGC TTG GCA ACA GAT
521
AKT-1 NM_005163.2 F -CCG ATT CAC GTA GGG AAA TG
R -AGC GTC GAA AAG GTC AAG TG
529
β-Catenin NM_001098209.1 F -GGT GGG CTG GTA TCT CAG AA
R -GGC AAC TGG TAA ACT GTC CAA
629
β-actin NM_001101.3 F -CTC CTG AGC GCA AGT ACT CC
R -ACA TCT CAA GTT GGG GGA CA
632

F and R indicates Forward and reverse sequences respectively