Table 1. Gene-specific primers along with their product size and gene bank accession number used for PCR for antiangiogenic activities determination.
Gene | Gene Bank Accession No. | Primer sequence | Size (bp) |
---|---|---|---|
VEGFR-2 | NM_002253.2 |
F -AGG TTG CGT GTT CTT CGA GT R -CCC AAA GTG CTG GGT TTT TA |
934 |
PI3K | NM_006218.2 |
F -CGT GTG CCA TTT GTT TTG AC R -TCA AAC CCT GTT TGC GTT TAC |
536 |
NF-kB | NM_001145138.1 |
F -TGG TCA GCT CCC TTC TCT GT R -GCC AGC TTG GCA ACA GAT |
521 |
AKT-1 | NM_005163.2 |
F -CCG ATT CAC GTA GGG AAA TG R -AGC GTC GAA AAG GTC AAG TG |
529 |
β-Catenin | NM_001098209.1 |
F -GGT GGG CTG GTA TCT CAG AA R -GGC AAC TGG TAA ACT GTC CAA |
629 |
β-actin | NM_001101.3 |
F -CTC CTG AGC GCA AGT ACT CC R -ACA TCT CAA GTT GGG GGA CA |
632 |
F and R indicates Forward and reverse sequences respectively