Table 2. Gene-specific primers used for adipogenesis and lipogenesis related PCR along with the product size and gene bank accession number details.
Gene | Gene bank accession no. | Primer sequence | Size (bp) |
---|---|---|---|
SREBP | NM_033218.1 |
F -TTG CAC CAG AGA GCA TTT TG-3’ R -GAA AAT GAG AGG CTG GTT GC-3’ |
593 |
C/EBPα | NM_007678.3 |
F -TTA CAA CAG GCC AGG TTT CC-3’ R -CCA CAG GGG TGT GTG TAT GA-3’ |
629 |
PPARγ | NM_001127330.1 |
F -CTG GCC TCC CTG ATG AAT AA-3’ R -GGG TGA AGG CTC ATG TCT GT-3’ |
393 |
aP2 | NM_024406.2 |
F -CAG CCT TTC TCA CCT GGA AG-3’ R -TCG ACT TTC CAT CCC ACT TC-3’ |
352 |
FAS | NM_007988.3 |
F -AAA GGA CCT GCC CAA TCT CT-3’ R -TGA TCA AAC TCA GGC TGC AC-3’ |
245 |
LPL | NM_008509.2 |
F -AAG CCC CAC AAG TGT AGT CG-3’ R -CGG ACA CAA AGT TAG CAC CA-3’ |
402 |
Actin | NM_007393.3 |
F-GTT GGT TGG AGC AAA CAT CC-3’ R-GAG GGT GAG GGA CTT CCT GT-3’ |
151 |
F and R indicates Forward and reverse sequences respectively