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. Author manuscript; available in PMC: 2018 Dec 6.
Published in final edited form as: Nature. 2016 Sep 14;537(7621):548–552. doi: 10.1038/nature19360

Extended Data Figure 4 |. H3K4me3 and DNA staining in oocytes, zygotes and cleavage-stage embryos.

Extended Data Figure 4 |

a, Panel from Fig. 1d with consistently adjusted brightness and contrast to allow visual observation of H3K4me3 signal following rapid loss at the late 2-cell stage. The signal of the strongly staining stages appears saturated due to this. b, c, Quantification of H3K4me3 and DNA staining by integrated H3K4me3-specific and DAPI-specific fluorescence intensity, respectively, over pronuclei and nuclei was determined using an epifluorescence microscope and the ImageJ software. The nuclear DNA content of ‘early’ and ‘late’ 2-cell embryos reveals that DNA synthesis is almost completed at the ‘early’ time point. Therefore suggesting an active mechanism of H3K4me3 removal rather than passive dilution over DNA replication (n = 18, 18, 20, 41, 18, 74 for respective time points; error bars show s.e.m.).