Skip to main content
. Author manuscript; available in PMC: 2019 Dec 5.
Published in final edited form as: Neuron. 2018 Oct 18;100(5):1163–1179.e4. doi: 10.1016/j.neuron.2018.09.048

Figure 5. Tak1 functions downstream of action-potential induced presynaptic calcium influx.

Figure 5.

(A) Representative traces for EPSC trains (scale, 50 nA, 40 ms). Stimulation frequency was 50 Hz, and extracellular calcium concentration was 1.5 mM. UAS-Tak1DN was expressed presynaptically with OK371-GAL4. (B) Average ratio of EPSC #4 in the train divided by EPSC #1 for the indicated genotypes. (C) Representative line scans and calcium transients (scale, 0.5 ΔF/F, 200 ms) for WT (grey bar) and Tak1179 (green bar). (D) Average ΔF/F for the indicated genotypes. (E) Representative calcium transients (scale, 1 ΔF/F, 400 ms) for the indicated genotypes. Stimulation was identical to that used in F. (F) Average ΔF/F for the indicated genotypes. (G) Cumulative frequency distributions of ΔF/F for WT (grey line) and Tak1179 (green line). (H) Average baseline fluorescence as a measure of dye-loading for the indicated genotypes. (I) Average decay constants of the transients in H and I for the indicated genotypes. Data are presented as average (+/− SEM) and statistical significance determined by Student’s t-test (unpaired, two-tailed) (D-I) or by one-way ANOVA with Tukey’s multiple comparisons test (B).