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. Author manuscript; available in PMC: 2019 Dec 5.
Published in final edited form as: Neuron. 2018 Nov 1;100(5):1059–1065.e4. doi: 10.1016/j.neuron.2018.10.011

Figure 3. Light-triggered and spontaneous waves have similar spatiotemporal properties and recruit the same retinal circuits.

Figure 3.

a. Summary data depicting wave propagation speed and wave propagation area for spontaneous and light-evoked waves. Notches: 95% CI.

b. Left: Pseudocolored representation of 164 RGCs responding to light onset (ON; green), light offset (OFF; magenta) or both (ON-OFF; white). Colored traces are example ∆F/F responses of individual RGCs to the flash of light. Right: example traces of spontaneous activity over 5 minutes for the same cells. Scale bar = 30 µm.

c. Left: RGCs were characterized by their light response (NR = no light response). Middle: Heatmap depicting max intensity of an RGC ∆F/F response (row) during each spontaneous and light-triggered wave (column). The rows are ordered based on light response. The columns are ordered based on similarity between waves. Right: Notched box plot representing leaf number distributions (top) or rank (bottom) of RGCs as they participate in spontaneous (blue) and light-triggered (red) waves. The leaf numbers come from a dendrogram that was generated using UPGMA hierarchical clustering of RGC activity profiles during spontaneous and light-triggered waves. Notches represent 95% CI. Rank is based on similarity to a model cell that responded equally to every wave. 0 is most similar.