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PCR primers for genotyping
| Primers | Sequences (5′→3′) |
|---|---|
| Pum2-F1 | GCTACTCCCTTTCTTGCTTG |
| Pum2-R1 | CCGTGAGTGAAAGAAATCTAAAC |
| Pum2-FrtR | CCCTTCCTCCTACATAGTTGGC |
| Pum2-F3 | GCACAGAAAAAAACCTTTGAAAGTC |
| Pum2-R2 | ATGGCAAAGCTCAAAATTCCACTT |
| Pum2-KOR | TTTGAACTGATGGCGAGCTC |
| Pum2-loxR | AAAACAACTATTAAATAACACCGCCTACTG |
RNA extraction and reverse transcription-PCR (RT-PCR)
