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RT-PCR primers for Pum2 mRNA
| Primers | Sequences (5′→3′) |
|---|---|
| exon4-F | AATGCAATTCTTTCTCCACG |
| exon6-F | GTAATCAGGTACCCATGGAT |
| exon7-R | TTAACTGCTGAACTGTTAGTG |
| exon8-R | GCAGCACTAATAATATATGGAT |
| exon10-R | AGCAAGACTCTGACCAAAAG |
| exon12-R | CATTTGTGCTGCCTGTAAGA |
Protein extraction and Western blot.
