Table 2.
Minimum Information Standard for Engineered Organism Experiments (MIEO) v0.1, with Group 3 factors and levels as an example (Fig. 1a)
| MIEO category | Factor | Level |
|---|---|---|
| Media components (R) | Yeast extract | 24 g L−1 |
| Yeast extract source | Sigma Y1625-250G, Lot SLBR9838V | |
| Glycerol | 5 g L−1 | |
| Magnesium sulfate | 0.12 g L−1 | |
| Tryptone | 12 g L−1 | |
| Potassium phosphate | 2.28 g L−1 | |
| Dipotassium phosphate | 12.7 g L−1 | |
| Sodium chloride | 6.63 g L−1 | |
| Water | DI water (18 MΩ-cm) | |
| Media properties (O) | pH | 7.2 |
| Buffer capacity | 90 mmol L−1 | |
| Osmolality | 750 mmol kg−1 | |
| Container geometry (R) | Type | 96-well plate |
| Well shape | Square | |
| Well bottom | Round | |
| Well volume | 2.5 mL | |
| Fill volume | 10% (0.25 mL) | |
| Cover | AeraSeal | |
| Container shaking (R) | Shaking speed | 460 rpm |
| Shaking diameter | 12.5 mm | |
| Shaking mode | Orbital | |
| Time (R) | Growth time | 48 h |
| Environment (R) | Temperature | 30 °C |
| Relative humidity | 80% | |
| Selective agents (O) | Antibiotic type | Chloramphenicol |
| Antibiotic concentration | 25 μg mL−1 | |
| Inoculum (R) | Concentration at inoculation | OD600 = 0.01 |
| Age of inoculum at inoculation | 16 h | |
| Inducers (O) | None | None |
The nine MIEO factor categories are given, along with whether their reporting is required (R) or optional (O). Factors and their level are reported