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. 2018 Dec 6;8:17686. doi: 10.1038/s41598-018-36083-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GAD expression in all layers of the parasagittal cortex in PG1 and PG2 of control (n = 5) and hypoxia-ischaemia (HI, n = 5) animals at 7 d recovery. Representative images of (A) control and (B) HI tissue in PG1 showing a reduction in GAD⁺ neurons (brown cells) in the HI cortex. GAD⁺ neurons in (B) are indicated with arrowheads. (C) Density of GAD⁺ neurons in PG1 and PG2 of control and HI animals. *p < 0.05. Scale bar: 50 µm. Data are mean ± standard deviation.