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. 2018 Dec 6;8:17686. doi: 10.1038/s41598-018-36083-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative examples of PNNs in the parasagittal cortex of control and HI animals at 7 d recovery. Control (left column), HI (right column). WFA (A,B: grey, C–H: red), Hoechst 33258 (blue). Note that because of the marked reduction in WFA staining in HI animals, the image intensity of injured tissues (D,F,H) was manually adjusted (four-fold increase relative to control images) to allow visualisation of staining patterns. Representative examples of actual WFA staining levels between control and HI animals are shown in panels A and B. Images (C–H) were taken from PG1. Images (E–H) were taken from layer 6. Arrowheads indicate PNNs. The diffuse extracellular WFA staining is observed within layer 6 indicated by dotted lines. Scale bars: (A,B) 5 mm, (C, D) 500 µm, (E, F) 100 µm, (G, H) 20 µm.