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. 2018 Dec 6;9:5207. doi: 10.1038/s41467-018-07568-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Synthesis and manipulation of monolayer dendrimicelle superstructures. a Complexation and reduction of Au(III) ions inside PAMAM dendrimers provides dendrimer-encapsulated gold nanoparticles (AuDENs). Mixing of these AuDENs with a negative-neutral pMAA₆₄PEO₈₈₅ block copolymer results in ~50 nm dendrimicelles. b For cryoTEM, micrometer-sized grid holes are filled with an aqueous thin dendrimicelle film, with the biconcave shape of this layer acting as a template for the organization of dendrimicelles into (sub-) micrometer-sized monolayer superstructures (I). Exposure of the superstructure to an electron beam results in evaporation of water (II). Prolonged exposure results in the rupture of the superstructure, with the embedded AuNPs revealing local movements of dendrimicelle building blocks as well as the global migration of dendrimicelles during the nanoexplosion (III). After the nanoexplosion, a hole similar in size to the superstructure is left behind (IV)