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. 2018 Dec 6;50(12):1–15. doi: 10.1038/s12276-018-0185-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Chemical structure of IM. b Dose-response curve of the inhibition of the ETC by IM in the A549 lung carcinoma cell line. Cells were treated with serially diluted IM for 24 h, and the basal OCR was measured using a Sea Horse XF Analyzer. c MEFs and d HFFs were reprogrammed into iPSCs with OSKM reprogramming factors in the presence of 10 nM IM or rotenone. Representative images of AP⁺ colonies are shown (top). The total number of AP⁺ colonies was counted on day 14 (D14, MEFs) or day 28 (D28, HFFs) of reprogramming (bottom). e mESCs cultured under the non-self-renewing conditions (−LIF) or self-renewing conditions (+LIF) were treated with 10 nM IM for 4 days. Representative images of AP⁺ colonies (top) and the total number of AP⁺ colonies are shown (bottom). f hESCs were maintained under the self-renewing condition (CM). hESCs cultured under non-self-renewing conditions (UM) were treated with 10 nM IM for 6 days. Representative images of AP⁺ colonies (top) are shown, and the relative AP expression was quantified by scanning densitometry (bottom). *p < 0.05; **p < 0.01; ***p < 0.001 (Student’s t-test)