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. 2018 Dec 6;8:17696. doi: 10.1038/s41598-018-35793-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of the agents on MP. Overnight grown MRSA CI cells were incubated with DiOC₂(3). From this mixture, 10 μl were added to 40 μl reaction containing agents at sub-MIC or PBS (untreated control) and read immediately in fluorimeter. Excitation was read at 450 nm and emission at 690 nm. The difference in fluorescence between tested compounds treated and untreated control cells was detected at 690 nm and calculated as a percentage compared to untreated control (100%). *Significantly lower than the value for the untreated control (P < 0.05).