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. 2018 Nov 30;9:721. doi: 10.3389/fendo.2018.00721

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Copyright © 2018 Chen, Yang, Han, Yang, Cen, Liu, Zhang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of intracellular signaling pathway inhibitors on GnRH-stimulated Gh release. The primary cultured pituitary cells of intersexual ricefield eels were pre-incubated for 24 h before being treated with 100 nM of GnRH1 (A) or GnRH3 (B) in the presence or absence of inhibitors Rp-cAMPS (50 μM), U73122 (10 μM), GF109203X (20 μM), Xestospongin C (1 μM), or Nifedipine (10 μM) for 4 h, respectively. After drug treatment, culture medium was harvested for measurement of Gh release by competitive ELISA. Data were expressed as fold change relative to the vehicle control. Bars represent means ± SEM (n = 3). ^*P < 0.05 vs.the vehicle control.