Table 1.
Percent amino acid identity matrix of PvGmGSTUG with the first 12 sequences identified in the BLASTP search.
| PvGmGSTUG | MtGS1 | MtGST2 | MtGST3 | TsPr1 | TsPr2 | CaGST1 | CaGST2 | MtGST4 | MtGST5 | TsPr3 | MtGST6 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1:PvGmGSTUG | 100.00 | 85.71 | 77.68 | 77.68 | 77.23 | 75.00 | 74.55 | 72.77 | 73.66 | 76.26 | 71.82 | 71.43 |
| 2:MtGST1 | 85.71 | 100.00 | 79.91 | 78.12 | 77.23 | 75.00 | 78.57 | 74.55 | 75.00 | 78.54 | 72.73 | 74.55 |
| 3:MtGST2 | 77.68 | 79.91 | 100.00 | 79.02 | 80.36 | 77.68 | 74.55 | 74.55 | 77.23 | 80.37 | 76.36 | 75.45 |
| 4:MtGST3 | 77.68 | 78.12 | 79.02 | 100.00 | 74.55 | 73.66 | 74.11 | 75.45 | 89.29 | 81.28 | 80.00 | 86.61 |
| 5:TsPr1 | 77.23 | 77.23 | 80.36 | 74.55 | 100.00 | 87.95 | 75.89 | 73.66 | 74.11 | 79.91 | 76.82 | 70.54 |
| 6:TsPr2 | 75.00 | 75.00 | 77.68 | 73.66 | 87.95 | 100.00 | 70.54 | 69.64 | 72.32 | 77.17 | 72.73 | 69.20 |
| 7:CaGST1 | 74.55 | 78.57 | 74.55 | 74.11 | 75.89 | 70.54 | 100.00 | 79.46 | 72.32 | 75.34 | 73.64 | 71.43 |
| 8:CaGST2 | 72.77 | 74.55 | 74.55 | 75.45 | 73.66 | 69.64 | 79.46 | 100.00 | 74.11 | 75.80 | 72.27 | 72.77 |
| 9:MtGST4 | 73.66 | 75.00 | 77.23 | 89.29 | 74.11 | 72.32 | 72.32 | 74.11 | 100.00 | 80.37 | 77.73 | 87.50 |
| 10:MtGST5 | 76.26 | 78.54 | 80.37 | 81.28 | 79.91 | 77.17 | 75.34 | 75.80 | 80.37 | 100.00 | 79.07 | 76.26 |
| 11:TsPr3 | 71.82 | 72.73 | 76.36 | 80.00 | 76.82 | 72.73 | 73.64 | 72.27 | 77.73 | 79.07 | 100.00 | 74.55 |
| 12:MtGST6 | 71.43 | 74.55 | 75.45 | 86.61 | 70.54 | 69.20 | 71.43 | 72.77 | 87.50 | 76.26 | 74.55 | 100.00 |
For the analysis, the amino acid sequence of PvGmGSTUG was used in the query. Percent identity matrix was calculated with the CLUSTAL Omega sequence alignment program (Sievers et al., 2011). The accession numbers of the GST sequences that resulted from the searches were: MtGST.1, (Medicago truncatula GST, XP_003623196.1); MtGST.2, (Medicago truncatula GST, XP_003623195.1); MtGST.3, (Medicago truncatula GST, XP_003623174.1); CaGST1, (Cicer arietinum GST3, ALZ41813.1); CaGST2, (Cicer arietinum GST, XP_004492376.1); MtGST4, (Medicago truncatula GST, XP_013449023.1); MtGST5, (Medicago truncatula GST, XP_003623168.1); MtGST6, (Medicago truncatula GST, XP_003623173.1); LaGST, (Lupinus angustifolius GST, XP_019459310.1); MtGST7, (Medicago truncatula GST, XP_003623171.2); GmGST1, (Glycine max GST, NP_001238439.1); CcGST, (Cajanus cajan GST, XP_020206357.1); GmGST2, (Glycine max GST, NP_001304556.1); GsGST1, (Glycine soja, KHN05112.1); and GsGST2, (Glycine soja, KHN06986.1). The GST isoenzymes and 100% identity are in bold.