Table 4.
Steady-state kinetic parameters of PvGmGSTUG for the CDNB/GSH substrate system (A) and for the CuOOH/GSH substrate system (B).
| Substrate system | Km (mM) (GSH) | S0.5 (mM) (CDNB) | kcat (min−1) (GSH) | nH (CDNB) | kcat/Km (mM−1 min−1) (GSH) | kcat/S0.5 (mM−1 min−1) (CDNB) |
|---|---|---|---|---|---|---|
| A | ||||||
| CDNB/GSH | 1.17 ± 0.09 | 0.88 ± 0.05 | 194.1 ± 4.85 | 1.77 ± 0.14 | 165.9 ± 0.14 | 217.5 |
| Substrate system | Km (mM) (GSH) | Km (mM) (CuOOH) | kcat (min−1) (GSH) | kcat/Km (mM−1 min−1) (GSH) | kcat/Km (m−1 min-1) (CuOOH) | |
| B | ||||||
| CuOOH/GSH | 0.017 ± 0.001 | 0.34 ± 0.02 | 29.61 ± 0.32 | 1,741.5 ± 127.9 | 87.1 ± 3.08 | |