Fig. 5.

Selective homing and engraftment of N3-ICtg DP thymocytes, highly expressing Notch3 and CXCR4. a (CD4⁺CD8⁺Notch3^+highCXCR4^+high) N3^high or (CD4⁺CD8⁺Notch3^+lowCXCR4^+high) N3^low thymocytes engraft in BM day10 post-intravenous injection (i.v.). Percentages of DP cells (middle) and of CD4⁺CD8⁺Notch3⁺CXCR4⁺ (right). b Absolute numbers of DP infiltrating NSG BM after N3^high (purple-circle) or N3^low (blue-square) thymocytes i.v. (upper, Kruskal–Wallis test) and the ratio N3^high/N3^lowDP cells at day1 (green-hexagon) and day10 (red-triangle) (lower, Mann–Whitney test) (*p<0.05). c Ex vivo experiments: percentages of AnnexinV⁺cells in N3^high (upper) and N3^low (lower) thymocytes in 24 h SDF-1-treated relative to saline (PBS). Data are represented as meanvalue ± SD (**p < 0.01; ns, not significant; Student’s t-test). d Absolute DP T-cell numbers in spleen after N3^high (purple-circle) or N3^low (blue-square) DP thymocytes i.v. (upper) and the ratio of N3^high/N3^lowDP T-cells (lower) at day1 (green-hexagon) and day10 (red-triangle) (*p<0.05; Student’s t-test, Mann–Whitney). See also Figure S3A and B