Fig. 7.

Comparative protein expression analysis of graft and host microglia in mouse and human chimeras. a, b Expression of host microglia-specific markers TMEM119 (a) and P2RY12 (b) in the cortex of mice that received lineage-negative BM carrying the lentiviral construct conferring eBFP expression. Host microglia (green) and donor cells (white) are indicated with arrowheads. IBA-1 immunohistochemistry for microglia (red). DAPI nuclear counterstain (blue). Scale bars, 30 µm. c, d Representative images of cortical, hippocampal, and cerebellar sections from female patients that received male donor BM grafts carrying the Y-chromosome (Y-chr). Host microglia are indicated by solid arrowheads and donor cells are shown by open arrowheads. Immunohistochemistry (red) of IBA-1 (c) and P2RY12 (d) combined with in situ hybridization of Y-chr (brown). Insets show a single grafted cell at higher magnification. Scale bars, 30 µm