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. 2018 Nov 30;12:885. doi: 10.3389/fnins.2018.00885

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Copyright © 2018 Ren, Tian, Zhao, Luo, Feng, Gong and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DTT increases the distribution fluorescence intensity of tdTomato during dehydration and embedding. (A) tdTomato-labeled VIP neurons in slices after dehydration without and with DTT (7 mM) (scale bar: 20 μm). (B) Fluorescent protein preservation ratios of neurons in dehydration agents containing different concentration of DTT (n = 7, 12, 12, 12, 9, and 8, respectively). Error bars represent SD. One-way ANOVA followed by Tukey’s post hoc tests (^∗p < 0.05, ^∗∗∗∗p < 0.001). (C) tdTomato-labeled VIP neurons in slices after being embedded without and with DTT (10 mM) (scale bar: 20 μm). (D) Fluorescent protein preservation ratios after embedding with different concentrations of DTT in the resin (n = 15, 19, 15, and 16, respectively). Error bars represent SD. One-way ANOVA followed by Tukey’s post hoc tests (^∗p < 0.05, ^∗∗∗∗p < 0.001).