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. 2018 Nov 30;12:885. doi: 10.3389/fnins.2018.00885

FIGURE 2.

FIGURE 2

Increasing the fluorescent intensity of tdTomato and decreasing the background fluorescence with lower polarization temperatures. (A) tdTomato fluorescence and background fluorescence at different temperatures (scale bar in left column: 20 μm; scale bar in right column: 500 μm). (B) Fluorescent protein preservation ratio and the background fluorescence rising rate at different temperature (each column filled with magenta n = 8, each column filled with black n = 3). Error bars represent SD. One-way ANOVA followed by Tukey’s post hoc tests (∗p < 0.05, ∗∗∗∗p < 0.001). (C) Fluorescence brightness of the tdTomato fluorescence (scale bar: 20 μm). (D) Enlarged images from C showing the fibers around the soma after embedding at different conditions (scale bar: 10 μm). (E) Fluorescence intensity plots for the lines labeled in (d1 and d2).