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. 2018 Nov 30;12:885. doi: 10.3389/fnins.2018.00885

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Copyright © 2018 Ren, Tian, Zhao, Luo, Feng, Gong and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Preservation of fluorescence after resin embedding. (A) The VIP::tdTomato samples in normal and optimized GMA resin (scale bar: 30 μm). (B) Fluorescent protein preservation ratio and the background fluorescence rising rate after embedding (n = 30, 29, 36, 36 from left to right). Error bars represent SD. One-way ANOVA followed by Tukey’s post hoc tests (^∗∗∗∗p < 0.001). (C) Fine structures were clearly detected with the optimized embedding method (scale bar in the first two columns: 20 μm; Scale bar in the last column: 50 μm).