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. 2018 Nov 30;12:885. doi: 10.3389/fnins.2018.00885

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Copyright © 2018 Ren, Tian, Zhao, Luo, Feng, Gong and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DsRed/mCherry/GFP/BFP can be well preserved using the optimized GMA embedding method. (A) Using viral tracing (AAV+RV), we labeled the GABAergic input neurons of M1 in VGAT-Cre mice. GFP represented the input neurons of M1 in the right hemisphere, DsRed represented the input neurons of M1 in the left hemisphere, and BFP showed the start neurons in M1. The fluorescence of each protein was clearly detected following the optimized embedding method (scale bar: 20 μm). (B) DsRed and mCherry signals after embedding (scale bar in top column: 20 μm; scale bar in bottom column: 5 μm). (C) Fluorescent proteins preservation ratios after optimized embedding (n = 18, 22, 16, 33 from left to right). Error bars represent SD.