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. 2018 Nov 30;9:2829. doi: 10.3389/fimmu.2018.02829

Figure 3.

Figure 3

MCs degranulation and cytokine release in response to C. albicans. (A) BMMCs degranulation in response to C. albicans was evaluated by the release of β-hexosaminidase and the synthesis of leukotrienes. No degranulation was observed after 30 min, 1 or 2 h. Data were analyzed with paired 2-way ANOVA. NS, statistically non-significant. (B) Stimulation of BMMCs with C. albicans induced a quick release of TNF-α, IL6, and IL13 (already detectable after 3 h) and of IL-4. C. albicans yeasts induced a more prominent cytokine release compared to hyphae. Data were analyzed with paired 2-way ANOVA. (C) Expression levels of tnfα, il6, il13, il4 were confirmed by qPCR after 3 h of co-culture. Data were analyzed by Kruskal Wallis test. Nd, non-detected. (D) Challenge of dectin-1−/− MCs with C. albicans resulted in an impaired release of TNF-α, IL-6, and IL-13 compared to WT controls. Data were analyzed with paired 2-way ANOVA and Tukey's multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.