Figure 5.

Candida albicans-infected MCs increase peritoneal macrophage crawling and promote their chemotaxis toward the infection. (A) Single cell tracks during 24 h chemotaxis experiment. Green tracks indicate single cells tracks moving toward the conditioned media, red tracks indicate cells moving away from the chemoattractant. Representative data of three independent experiments. (B) Albeit failing to attract macrophages, culture supernatants from BMMCs + C. albicans co-culture increased macrophages crawling ability. Data were analyzed with ordinary one-way ANOVA and Tukey's multiple comparison test. (C) Transwell migration assay was set up against a live C. albicans-MCs infection (live cells) or against the same conditioned media (C.M.). While the live infection induced a prominent migration of macrophages, conditioned media were able to induce macrophage chemotaxis only partially. MCP-1 was used as positive control of migration. Bars represent mean ± SD of three independent experiments. Data were analyzed with one-way ANOVA and Tukey's multiple comparison test. (D) Representative images of migrated macrophages, stained by crystal violet. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001.