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. 2018 Nov 30;8:420. doi: 10.3389/fcimb.2018.00420

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Copyright © 2018 Chen, Guo, Shi, Fang, Chen and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Two clusters were observed in ascitic fluids microbiota. (A) The principal coordinate analysis of the Jensen-Shannon distance generated from the OTU-level relative abundance profiles. Samples are colored by clusters identified by the partitioning around medoids clustering algorithm. Dark blue, Cluster 1; red, Cluster 2. (B) Two clusters were supported with the highest Calinski-Harabasz (CH) pseudo F-statistic value, as the optimal number of clusters. (C) Boxplot comparison of the number of observed OTUs between Cluster 1 and Cluster 2. (D) Boxplot comparison of the Chao1 index between Cluster 1 and Cluster 2. **p < 0.01 based upon Mann-Whitney U-tests with Benjamini & Hochberg correction.