FIGURE 5.

Effects of goat cathelicidins, tachyplesin-1, and conventional antibiotics at different concentrations on the fluorescence resulting from the in vitro translation of EGFP with the use of E. coli BL21 (DE3) Star cell extract (A). Data are the mean ± SD of at least three independent experiments performed in triplicate. ChMAP-28 (B) and mini-ChBac7.5Nα (C) binding to DNA was examined by electrophoretic mobility shift assay (EMSA). Various amounts of the peptides were incubated with 100 ng of the pUC19 plasmid DNA, and DNA binding was assessed by the peptide influence on the electrophoretic mobility of DNA. DNA-to-peptide weight ratios are indicated on the horizontal axis. Lane M shows the DNA molecular size marker (500–10,000 bp).